Laboratory Technician - Advanced Omics Platform

Offer Description

We are looking for a Laboratory Technician to be part of the Advanced Omics Platforms (AOP) of Botton-Champalimaud Pancreatic Cancer Centre, to provide technical services to support the current operation.
 

Key Responsibilities

- Perform laboratory protocols to produce reliable and precise data to support scientific research.

- Perform routine laboratory protocols in accordance with Standard Operating Procedures (SOPs).

- Handle fresh and archival (fresh frozen and FFPE) from patient material.

- Prepare high quality DNA and RNA from human specimens and samples according to SOPs.

- Perform quality control of DNA and RNA preparations according to SOPs.

- Perform data entry and sample tracking using Laboratory Information Management Systems (LIMS).

- Producing accurate reports on DNA and RNA sample quality control.

- Preparation of Next Generation Sequencing (NGS) libraries.

- Troubleshoot and optimise library preparation protocols.

- Operate and maintain NGS platforms (e.g., Illumina, Oxford Nanopore and 10X Genomics) and related equipment.

- Efficiently resolve and document technical issues in a timely and clear manner.

- Develop positive relationships with internal teams and stakeholders.

- Ensure the care and cleanliness of the AOP designated lab space so as to comply with Quality System Regulation requirements.

- To work with other members of the AOP to help maintain laboratory stocks of kits, reagents and other consumable requirements.

- Participate in routine lab meetings and contribute to the planning and execution of research projects.

Requirements

Research Field: Biological sciences
Education Level: Master Degree or equivalent

Skills / Qualifications

- Leadership

- Problem solving

- Flexibility

- Attention to Detail

- Communication

- Organisation

- Initiative

Essential Qualifications:

- BSc and MSc or other post-graduate level qualification in life or medical sciences

- Good scientific knowledge with demonstrated ability to perform routine laboratory protocols accurately and efficiently.

- Experience in extracting DNA and RNA from fresh frozen or fixed material.

- Demonstrated experience in using standard molecular techniques including, cloning and PCR.

- Experience in following SOPs and following experimental workflows accurately and efficiently.

- Experience in using and analysing the results obtained from PCR and Real-time PCR machines.

- General IT proficiency with an ability to generate basic reports.

- Good communication and organisational skills.

- Proficient in both English and Portuguese

Specific Requirements / Desirable Qualifications

- Experience in NGS library preparation, either manually or using automated liquid handling robotics.

- Experience in running and maintaining NGS instruments such as Illumina’s NextSeq2000.

- Experience in handling patient specimens and/or samples.

- Experience in data entry and sample tracking using LIMS.

- Experience in using TapeStation or equivalent platform for assessing DNA and/or RNA quality.

- Experience with document management systems.

STANDARDS OF PERFORMANCE

Proactively looking for opportunities to improve service provision and subsequent delivery on the improvements identified. All individuals are expected to be flexible in undertaking the duties and responsibilities attached to their job and may be asked to perform other duties which reasonably correspond to the general character of their job and their level of responsibility.
All individuals are expected to maintain personal and professional development to meet the changing demands of the job and participate in appropriate learning and development activities.

Specific Requirements
 

- Internal Key Relationships 

- Head of Laboratory Operations

- Health&Safety Officer

- Research Group Leaders

- Advanced technology Heads

- IT

- HR
 

External Key Relationships

- Company service engineers.

- Company sales reps.

Languages

ENGLISH
Level: Good

Eligibility criteria / How to apply

SELECTION COMMITTEE
Craig Nourse (Manager Advanced Omics Platform)
Inês Sousa (Senior Technician Advanced Omics Platform)
Peter Bailey (Director of Translational Research)

Evaluation and Selection Criteria: 

All CVs and motivation letters will be reviewed by the selection committee. Reference letters will be requested from shortlisted candidates, and those candidates deemed particularly well suited for the position will be invited for an interview.

Candidates must provide a single PDF file named "FirstNameSurname_LT_AOP_BCPCC" to "recruitment.botton@research.fchampalimaud.org" containing:

A letter of motivation (max. 2 pages) highlighting your suitability for the role, salary expectations and notice period;
CV (max. 2 pages);
Names and contact of at least 2 references. If you do not wish that we contact current or past employers, please state so and explain why in your letter of motivation. 

About Us

The Botton-Champalimaud Pancreatic Cancer Centre is a state-of-the-art facility comprising research laboratories, a day hospital, a surgical centre, as well as inpatient and intensive care facilities. The mission of the Botton-Champalimaud Pancreatic Cancer Centre is to develop new and effective treatments for pancreatic cancer. To accomplish this goal a multidisciplinary team of surgeons, clinicians, clinical trials specialists, cancer scientists, genomics experts and computational biologists work together to deliver world-leading outcomes for patients with pancreatic cancer.

The Botton-Champalimaud Pancreatic Cancer Centre is also closely integrated with the Champalimaud Centre for the Unknown. Members of the Champalimaud Research Programme (CR) and the Champalimaud Clinical Centre (CCC), work together to investigate fundamental biological processes and search for effective solutions to alleviate the burden of oncological, neurological and mental diseases, while providing state-of-the-art care to the patients of the CCC. With 479 members, including group leaders, postdocs, PhD and MSc students, research technicians and highly specialised research staff, the CR community encompasses 39 nationalities.

Within the Lisbon Ocean Campus, CF will expand its research, technological development, innovation, and clinical practice in the digital therapeutics field.
 

Additional Institutional Information

In line with our community guidelines and Equality, Diversity and Inclusion policy and Gender Equality Plan, no candidate may be privileged, favoured, prejudiced or deprived of any right or excluded from any duty on the basis of age, sex, gender identity, sexual orientation, race/ethnicity, disability, chronic illness, language, nationality, territory of origin, family status (marital, pregnancy and maternity, having or not having dependents), socioeconomic situation, education, religion, political or ideological beliefs, trade union membership, or on any other grounds which are irrelevant to decision-making.

Legal Notices

The present call has the sole purpose of filling the indicated research position. This call may be cancelled at any time on or before the jury’s evaluation of all eligible candidates and the recruitment process shall be concluded on the day of the full execution of the contract between the Champalimaud Foundation and the selected candidate.